Interrogating molecular scale organization in planar lipid bilayers and liposomes. Monika J Dominska

ISBN: 9781109414769

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NOOKstudy eTextbook

168 pages


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Interrogating molecular scale organization in planar lipid bilayers and liposomes.  by  Monika J Dominska

Interrogating molecular scale organization in planar lipid bilayers and liposomes. by Monika J Dominska
| NOOKstudy eTextbook | PDF, EPUB, FB2, DjVu, talking book, mp3, RTF | 168 pages | ISBN: 9781109414769 | 3.33 Mb

Biomolecules such as membrane proteins and enzymes are attractive candidates for use in biosensing devices because of their high selectivity, but to maintain the biological activity of these biomolecules, the interface in which they reside must mimicMoreBiomolecules such as membrane proteins and enzymes are attractive candidates for use in biosensing devices because of their high selectivity, but to maintain the biological activity of these biomolecules, the interface in which they reside must mimic their natural environment.

The activities of biomolecules associated with biological membranes are best maintained in lipid bilayers. However, to make a sensing device, artificial lipid bilayers must be attached to a transducer surface. Because the organization of lipids and biomolecules in a biological membrane is a complex mosaic that is characterized by its fluidity, the biomimetic lipid bilayer structures on transducer surfaces must have fluid properties similar to those of biological membranes.-We have imbedded selected molecular probes into our biomimetic interfaces to study their properties as candidates for supported lipid membranes capable of maintaining the activity of biomolecules such as transmembrane proteins.

This research focuses on the organization of biomimetic interfaces absent protein incorporation. Our model systems were monolayers and bilayers doped with a reporter molecules. We have used self-assembly, Langmuir-Blodgett (LB), and Langmuir-Schaefer (LS) methods to construct planar lipid membranes on hydrophilic substrates (gold and indium-doped tin oxide (ITO) for electrochemistry, silica for spectroscopy).-Pyrene tethered to the interface acted as our reporter molecule.

This probe has shown to experience significant molecular freedom as the only constituent of the interface. The addition of aliphatic coadsorbates to the interface improves the organization of the molecules by making the interface more rigid. Capping the monolayer with top lipid leaflet helps the organization of the molecules in the bottom leaflet even further.-We attempted liposome fusion on gold surfaces where the liposomes were composed of mixed lipids including thio-lipids modified with polyethylene glycol (PEG) in the headgroup.

The failure to produce surface-attached lipid bilayer structures led to the need to understand the solution phase lipid assemblies that were presented to the interface. The addition of the thio-PEG lipid to the lipid assembly produces changes in organization that are most pronounced in the lipid headgroup region whereas the acyl chain region is minimally affected. To probe the dynamics of the acyl chain region we have used a free chromophore (perylene).



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